Skip to main content

Main menu

  • About the Journal
    • Aims & Scope
    • Editorial Board
    • Browse Archive
    • Abstracting - Indexing
    • About IWA Publishing
  • Subscribe
    • Institutional Subscriptions
    • User Licences
    • IP Registration
    • IWA Member Subscription
  • Open Access
  • For Authors
    • Online Submission
    • Publish with Us
    • Instructions for Authors
    • Open Access
    • How to review a paper
    • Rights & Permissions
    • Article Promotion
  • For Librarians
    • Usage Statistics
    • Subscriber Services
    • Sample Issue
    • Terms and Conditions
  • For Readers
    • Recommend to Your Library
    • Rights & Permissions
    • How to Subscribe
  • Collections
  • Help
    • FAQ
    • Contact Us
  • Other Publications
    • IWAP Online
    • Journal of Hydroinformatics
    • Journal of Water and Health
    • Journal of Water and Climate Change
    • Journal of Water Reuse and Desalination
    • Journal of Water Supply: Research and Technology-AQUA
    • H2Open Journal
    • Hydrology Research
    • Water Practice and Technology
    • Water Research
    • Water Policy
    • Water Quality Research Journal
    • Water Science and Technology
    • Water Science and Technology: Water Supply
    • Journal of Water Sanitation and Hygiene for Development
    • Water Intelligence Online
    • Ingeniería del agua
    • IWA Publishing

User menu

  • Log-in
  • Sign-up for alerts

Search

  • Advanced search
Water Science and Technology: Water Supply
  • Other Publications
    • IWAP Online
    • Journal of Hydroinformatics
    • Journal of Water and Health
    • Journal of Water and Climate Change
    • Journal of Water Reuse and Desalination
    • Journal of Water Supply: Research and Technology-AQUA
    • H2Open Journal
    • Hydrology Research
    • Water Practice and Technology
    • Water Research
    • Water Policy
    • Water Quality Research Journal
    • Water Science and Technology
    • Water Science and Technology: Water Supply
    • Journal of Water Sanitation and Hygiene for Development
    • Water Intelligence Online
    • Ingeniería del agua
    • IWA Publishing

Log-in

Sign-up for alerts   

  • My Cart
Water Science and Technology: Water Supply
Browse Archive
Advanced Search
  • About the Journal
    • Aims & Scope
    • Editorial Board
    • Browse Archive
    • Abstracting - Indexing
    • About IWA Publishing
  • Subscribe
    • Institutional Subscriptions
    • User Licences
    • IP Registration
    • IWA Member Subscription
  • Open Access
  • For Authors
    • Online Submission
    • Publish with Us
    • Instructions for Authors
    • Open Access
    • How to review a paper
    • Rights & Permissions
    • Article Promotion
  • For Librarians
    • Usage Statistics
    • Subscriber Services
    • Sample Issue
    • Terms and Conditions
  • For Readers
    • Recommend to Your Library
    • Rights & Permissions
    • How to Subscribe
  • Collections
  • Help
    • FAQ
    • Contact Us

You are here

  • Home
  • Archive
  • Volume 13, Issue 2

Efficacy of measuring cellular ATP levels to determine the inactivation of pulsed UV treated Cryptosporidium parvum oocysts suspended in water

Mary Garvey, Jennifer Hayes, Eoghan Clifford, Dominik Kirf, Neil Rowan
Published March 2013, 13 (2) 202-213; DOI: 10.2166/ws.2013.010
Mary Garvey
Department of Nursing and Health Science, Athlone Institute of Technology, Ireland E-mail: mgarvey@ait.ie
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jennifer Hayes
Department of Nursing and Health Science, Athlone Institute of Technology, Ireland E-mail: mgarvey@ait.ie
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eoghan Clifford
Department of Civil Engineering, National University of Ireland, Galway, Ireland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dominik Kirf
Department of Nursing and Health Science, Athlone Institute of Technology, Ireland E-mail: mgarvey@ait.ie
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Neil Rowan
Department of Nursing and Health Science, Athlone Institute of Technology, Ireland E-mail: mgarvey@ait.ie
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

This constitutes the first study to report on the use of a novel approach to determine inactivation in PUV-irradiated Cryptosporidium parvum oocysts suspended in water based on the measurement of cellular adenosine triphosphate (ATP) concentration. This study also compares the efficiency of a novel ATP assay to that of using the combined in vitro HCT-8 cell culture – quantitative polymerase chain reaction (qPCR) method for determining the inactivation in the waterborne pathogen C. parvum after exposure to pulsed UV (PUV) treatments. Findings were compared with using the combined cell culture-qPCR approach for determining oocyst viability in similarly treated samples. PUV effectively killed C. parvum with a 5.4 log10 loss in oocyst viability after exposure to a UV dose of 8.5 μJ/cm2 as determined by the in vitro cell culture – qPCR assay. The ATP assay was shown to be significantly less effective in measuring loss of oocyst viability in similarly PUV-irradiated samples for all combination of treatment regimes studied. Measurement of cellular ATP is not suitable as an indicator of the disinfection efficiency of PUV-irradiated C. parvum oocysts. The levels of ATP present post PUV-irradiated samples suggests that significant cellular activity remained in treated oocysts that are unable to invade human HCT-8 cells. However, further studies are merited to investigate factors that might aid repair post PUV treatments in this water-borne human parasite. Use of this ATP assay offers an interesting insight into loss of infectivity in PUV-treated C. parvum. This rapid assay does not appear suitable for investigating or optimizing treatment efficiency under varying operational settings as it detects PUV-treated oocysts at levels significantly higher compared with using the in vitro cell culture-qPCR infectivity assay. Overestimation of survivors by the ATP assay may suggest that a sub-population of C. parvum oocysts may exist in a viable but non-infectious state or may require a period of resuscitation to facilitate photo-repair (if possible) that may lead to regained ability to infective human hosts.

  • ATP assay
  • combined cell culture-qPCR
  • Cryptosporidium
  • pulsed UV light
  • viability
  • Received March 29, 2012.
  • Accepted August 24, 2012.
  • © IWA Publishing 2013

Log in using your username and password

Forgot your user name or password?

Purchase access

User Login Menu

  • Create a new account
  • Forgot username/password?
  • Can't get past this page?
  • Help with Cookies
  • Need to Activate?
Previous ArticleNext Article
Back to top

SELECTED ISSUE

Water Science and Technology: Water Supply: 18 (2)
  Volume 13, Issue 2

  Table of Contents
  Uncorrected Proofs
  Browse Archive

Actions

Email

Thank you for your interest in spreading the word on Water Science and Technology: Water Supply.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Efficacy of measuring cellular ATP levels to determine the inactivation of pulsed UV treated Cryptosporidium parvum oocysts suspended in water
(Your Name) has sent you a message from Water Science and Technology: Water Supply
(Your Name) thought you would like to see the Water Science and Technology: Water Supply web site.
Share
Efficacy of measuring cellular ATP levels to determine the inactivation of pulsed UV treated Cryptosporidium parvum oocysts suspended in water
Mary Garvey, Jennifer Hayes, Eoghan Clifford, Dominik Kirf, Neil Rowan
Water Science and Technology: Water Supply Mar 2013, 13 (2) 202-213; DOI: 10.2166/ws.2013.010
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Connotea logo Facebook logo Google logo Mendeley logo
Citation Tools
Efficacy of measuring cellular ATP levels to determine the inactivation of pulsed UV treated Cryptosporidium parvum oocysts suspended in water
Mary Garvey, Jennifer Hayes, Eoghan Clifford, Dominik Kirf, Neil Rowan
Water Science and Technology: Water Supply Mar 2013, 13 (2) 202-213; DOI: 10.2166/ws.2013.010

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
View Full PDF
Save to my folders
Alerts

Please log in to add an alert for this article.

  • Tweet Widget
  • Facebook Like

Jump to

  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Hydraulic optimization of corrugated stilling basin with adverse slope
  • The origin and risks associated with loose deposits in a drinking water distribution system
  • Analysis of carbon emission hot spot and pumping energy efficiency in water supply system
Show more Research Article

Similar Articles

Keywords

ATP assay
combined cell culture-qPCR
Cryptosporidium
pulsed UV light
viability
  • Current Issue
  • Uncorrected Proofs
  • Browse Archive
  • Feedback
  • Online Submission
  • Subscribe
  • Contents Alerts
  • About the Journal
  • Open Access
  • Rights & Permissions

IWA Publishing
Alliance House
12, Caxton Street
London SW1H 0QS, UK

Tel: +44 (0)20 7654 5500
Fax: +44 (0)20 7654 5555
Remove (0) if calling from outside the UK
iwapublishing.com
Company registered in England no. 3690822

© IWA Publishing | Cookies | Terms & Conditions | Site Map | Privacy | ISSN Print: 1606-9749 | ISSN Online: 1607-0798